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Item #:
149738059
DPBS Buffer 1X, 1000ml Dulbecco's Phosphate-Buffered Saline Without Calcium & Magnesium, pH 7.4, Sterilization, 0.1um Filter, 1000ml
Item #:
149738059
GMD 2175
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Ideal for cell dissociation and other procedures requiring controlled ion conditions, ensuring consistent and reliable results.
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What Stands Out
Sterile Formula
The DPBS Buffer is sterilized using a 0.1um filter, ensuring a contamination-free solution ideal for sensitive biological applications and experiments.
Calcium & Magnesium-Free
This buffer is free from calcium and magnesium, making it suitable for various cell culture applications, particularly for cells sensitive to these ions.
Optimal pH
Maintaining a stable pH of 7.4, this buffer provides an optimal environment for cell survival and function, ensuring reliable results in laboratory conditions.
Product Details
| Item Weight | 2.2 lbs (1 kg) |
Who Should Buy?
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Cell Culture
Ideal for maintaining and washing mammalian cells due to its balanced ionic composition and pH stability.
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Laboratory Research
Suitable for various molecular and cellular biology experiments requiring isotonic conditions without calcium and magnesium.
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Tissue Samples
Effective for resuspending and transporting tissue samples while preventing cell lysis.
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Calcium-Dependent Cells
Not suitable for experiments requiring calcium or magnesium ions for cellular activity or signaling.
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Clinical Applications
Not recommended for direct use in clinical settings or procedures involving human patient samples.
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Non-sterile Processes
May not fit applications where complete sterility is critical beyond what is provided by filtration.
Product Description
DPBS Buffer 1X, 1000ml Dulbecco's Phosphate-Buffered Saline Without Calcium & Magnesium, pH 7.4, Sterilization, 0.1um Filter, 1000ml
Have any Query? Chat with us
Customer Questions & Answers
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Question:
What is DPBS Buffer 1X?
Answer: DPBS Buffer 1X, or Dulbecco's Phosphate-Buffered Saline, is a balanced salt solution widely used in biological and laboratory applications. It provides essential ions and maintains physiological pH, which is crucial for cell culture, washing cells, or diluting biological samples. Without calcium and magnesium, this formulation is ideal for experiments where these ions might interfere, ensuring accurate results during sensitive experiments. -
Question:
How is DPBS Buffer 1X used in cell culture?
Answer: DPBS Buffer 1X is extensively utilized in cell culture practices to rinse cells prior to trypsinization or to remove serum from the culture medium. This gentle solution helps maintain osmotic balance and prevents cell lysis during handling. Ideal for sensitive cell types, it ensures cells are treated with the utmost care while preparing for experiments or analysis. -
Question:
Is DPBS Buffer 1X sterile?
Answer: Yes, the DPBS Buffer 1X is sterilized through a 0.1µm filter, ensuring that it is free from contaminants. The sterilization process is crucial in laboratory settings where maintaining aseptic conditions is necessary for reliable results. Using sterile DPBS minimizes the risk of microbial contamination during cell culture and other sensitive laboratory procedures. -
Question:
What is the significance of pH 7.4 in DPBS Buffer 1X?
Answer: The pH of 7.4 in DPBS Buffer 1X is physiologically relevant and mimics the natural environment of human tissues. This pH level is critical for maintaining cellular functions and ensuring optimal reactions during experiments. Using a buffer that maintains this neutral pH is essential for any studies involving live cells or biological samples to reflect physiological conditions accurately. -
Question:
Can DPBS Buffer 1X be used for washing cells?
Answer: Absolutely! DPBS Buffer 1X is often used for washing cells before further processing or analysis. By removing unwanted substances or leftover media, it prepares the cells for experiments such as flow cytometry, immunostaining, or any assay where cellular integrity is important. This washing step is vital to ensure that only the targeted cells and techniques are utilized, improving the accuracy of results. -
Question:
What is the difference between DPBS with and without calcium and magnesium?
Answer: DPBS Buffer 1X without calcium and magnesium is specifically formulated for situations where these ions might interfere with protocols, such as cell detachment or trypsinization. By omitting these ions, researchers can prevent unwanted cellular interactions and ensure better cell viability post-experiment. This makes it an essential choice for researchers focusing on cellular assays or sensitive preparations. -
Question:
Is DPBS Buffer 1X suitable for in vitro experiments?
Answer: Yes, DPBS Buffer 1X is highly appropriate for various in vitro experiments including cell culture, enzyme studies, and reconstitution of protein solutions. Its balanced ion composition and physiological pH make it optimal for maintaining cell health and promoting stable experimental conditions. Researchers use it extensively due to its versatility across different biological protocols. -
Question:
What kinds of experiments is DPBS Buffer 1X commonly used in?
Answer: DPBS Buffer 1X finds application in experiments like cytotoxicity assays, immunofluorescence, and enzyme activity assays. Due to its gentle handling properties, it is favored for preparing and washing cells without compromising integrity. Labs utilize it for accurate biological assays, ensuring that cells are treated in a stable, physiologically relevant environment. -
Question:
How is DPBS Buffer 1X stored?
Answer: To maintain its efficacy, DPBS Buffer 1X should be stored at 2-8°C in a sealed container to prevent contamination. Once opened, it’s advisable to use it within a suitable timeframe to ensure sterility. Proper storage is crucial in retaining the buffer's quality and reliability for experiments, providing consistent results across various applications. -
Question:
Where can I buy DPBS Buffer 1X, 1000ml in The Gambia?
Answer: You can purchase DPBS Buffer 1X, 1000ml from Ubuy in The Gambia. Ubuy offers a variety of laboratory supplies, ensuring you have access to high-quality products conveniently. Their platform enables easy ordering and prompt service that caters to your laboratory needs, streamlining your purchasing process for essential research materials.
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GMD 2175
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Features & Benefits
- DPBS Buffer 1X, 1000ml, designed for sensitive experiments.
- Contains no calcium or magnesium ions to prevent interference.
- Sterilized via 0.1um filter for low endotoxin levels.
- Packaging reduces exposure to air and maintains quality.
- Suitable for cell/tissue sample transportation and pre-wash.
- Long shelf life of at least 3 years when stored at room temperature.
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