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- ABI RPMI 1640-PenStrep, HEPES, L-Gln, 500 mL
ABI RPMI 1640-PenStrep, HEPES, L-Gln, 500 mL
GMD 6515
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RPMI 1640 medium is suitable for the culture of mammalian myeloma and hybridoma.
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Product Details
| Item Weight | 1.1 lbs (500 grams) |
Who Should Buy?
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Cell Culture Labs
Ideal for laboratories engaging in mammalian cell culture, providing necessary nutrients and antibiotics for optimal growth.
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Biotech Research
Supports research requiring primary cell lines and ensures a controlled environment for biological experiments.
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Tissue Engineering
Beneficial for applications in tissue engineering requiring stable pH and nutrient supply for cell viability.
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Aquaculture Use
Not suitable for aquatic applications or environments focusing on the cultivation of fish or aquatic organisms.
Product Description
ABI RPMI 1640-PenStrep, HEPES, L-Gln, 500 mL
About This Item
Introducing the RPMI 1640-PenStrep, HEPES, L-Gln, 500 mL Discover the ultimate solution for mammalian cell culture with our RPMI 1640-PenStrep, HEPES, L-Gln, 500 mL medium. Named after the prestigious Roswell Park Memorial Institute, this high-quality formulation is developed to ensure optimal cell growth and viability. Perfect for a wide range of applications, including the culture of mammalian myeloma and hybridoma, our RPMI 1640 medium is designed to meet the specific needs of researchers and scientists working with mouse hybridomas, human leukocytes, B and T lymphocytes, as well as suspension cultures or monolayer human leukemic cells. With our RPMI 1640 medium, you can expect exceptional results no matter the nature of your experiment or study. The inclusion of Glutamine, Penicillin, and Streptomycin ensures a highly supportive environment for your cell cultures, promoting cell growth and minimizing the risk of contamination. When it comes to sourcing your laboratory supplies, convenience is key.
That's why we offer a seamless online shopping experience through our trusted platform, Ubuy. Whether you're a seasoned researcher or a first-time buyer, you can easily purchase the RPMI 1640-PenStrep, HEPES, L-Gln, 500 mL right from the comfort of your own lab. Don't miss out on the opportunity to streamline your cell culture process. Order the RPMI 1640-PenStrep, HEPES, L-Gln, 500 mL today and experience unmatched quality and convenience with Ubuy.
Customer Questions & Answers
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Question:
What is ABI RPMI 1640-PenStrep, HEPES, L-Gln used for?
Answer: ABI RPMI 1640-PenStrep, HEPES, L-Gln is a culture medium widely utilized in biological research and clinical labs for the growth of various cell types, including hematopoietic cells. This formulation enhances cell viability and proliferation by providing essential nutrients and supplements. Additionally, it includes antibiotics (Penicillin and Streptomycin) to prevent bacterial contamination, HEPES to maintain stable pH, and L-Glutamine as an amino acid necessary for protein synthesis. It's frequently used in cell culture applications, drug testing, and studies involving cancer cells. -
Question:
Is ABI RPMI 1640-PenStrep, HEPES, L-Gln suitable for primary cell cultures?
Answer: Yes, ABI RPMI 1640-PenStrep, HEPES, L-Gln is suitable for primary cell cultures. The formulation is specifically designed to support the growth of a wide range of cell types, including those derived from primary tissues. Its rich nutrient composition allows primary cells to thrive, making it an ideal choice for researchers conducting experiments that require fresh cell lines. This medium is particularly beneficial when performing assays, drug evaluations, or any work involving specific cell types derived from human or animal sources. -
Question:
How should ABI RPMI 1640-PenStrep, HEPES, L-Gln be stored?
Answer: ABI RPMI 1640-PenStrep, HEPES, L-Gln should be stored in a cool, dry place, preferably in a refrigerator at 2-8°C. This storage helps maintain the stability of the nutrients and supplements present in the medium. Avoiding exposure to extreme temperatures or direct sunlight is essential to prevent the degradation of its components. For laboratory use, it is advisable to reconstitute the medium as needed and utilize it promptly for optimal cell culture performance. -
Question:
Can ABI RPMI 1640-PenStrep, HEPES, L-Gln be used for serum-free cultures?
Answer: While ABI RPMI 1640-PenStrep, HEPES, L-Gln is traditionally used with serum supplements, researchers often adapt it for serum-free cultures by adding growth factors or supplements specific to their cell type. The absence of serum makes it ideal for applications requiring controlled variables. When transitioning to serum-free cultures, consider optimizing concentrations of additional supplements to ensure cell health and proliferation. This medium enables versatile culture systems in various experimental settings. -
Question:
What cell types can I culture using ABI RPMI 1640-PenStrep, HEPES, L-Gln?
Answer: You can culture a variety of cell types with ABI RPMI 1640-PenStrep, HEPES, L-Gln, particularly lymphocytes, hybridomas, and certain tumor cell lines. The balanced formulation supports the metabolic needs of these cells, allowing for effective growth and maintenance. This versatility makes it a popular choice in immunology, cancer research, and regenerative medicine. Researchers frequently find it useful for studies concerning immune responses and cellular interactions during experimental procedures. -
Question:
What is the importance of L-Glutamine in ABI RPMI 1640-PenStrep, HEPES, L-Gln?
Answer: L-Glutamine plays a critical role in ABI RPMI 1640-PenStrep, HEPES, L-Gln as it aids in protein synthesis and cellular metabolism. As an essential amino acid, it serves as a nitrogen source for various biosynthetic processes and supports cell proliferation. Its presence in the medium ensures that cultured cells can sustain their growth and maintain normal metabolic activity. L-Glutamine is especially important in cultures experiencing rapid cell division, like lymphocytes in immune assays or tumor cell research, making this medium very effective. -
Question:
Can ABI RPMI 1640-PenStrep, HEPES, L-Gln be used in drug testing?
Answer: Yes, ABI RPMI 1640-PenStrep, HEPES, L-Gln is frequently used in drug testing and pharmacological studies, particularly for cancer therapeutics and immunomodulation assessments. The medium's formulation supports cell viability, allowing researchers to assess the efficacy and toxicity of drugs in a controlled environment. Its use in screening assays enables scientists to observe cellular responses to various treatments, making it an essential tool in drug discovery processes and therapeutic development. -
Question:
How do I prepare ABI RPMI 1640-PenStrep, HEPES, L-Gln for use?
Answer: To prepare ABI RPMI 1640-PenStrep, HEPES, L-Gln for use, you need to ensure it is thoroughly mixed before transferring it to your cell culture vessel. If using it for the first time or after storage, gently invert the bottle to resuspend any settled components. Depending on your protocol, you may supplement the medium with serum or other growth factors before addition to cultured cells. Always perform a pH check and other necessary assessments to ensure the medium is optimized for your specific cell culture requirements. -
Question:
Where can I buy ABI RPMI 1640-PenStrep, HEPES, L-Gln in The Gambia?
Answer: You can buy ABI RPMI 1640-PenStrep, HEPES, L-Gln from Ubuy, which is a reliable e-commerce platform offering a wide variety of laboratory supplies. Ubuy provides an efficient shopping experience and specializes in delivering products tailored for research and scientific purposes. By visiting Ubuy, you can easily find and acquire this essential cell culture medium, ensuring you have quality supplies for your experiments and testing.
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GMD 6515
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Features & Benefits
- Specifically formulated for culturing mouse hybridomas, human leukocytes, and B and T lymphocytes
- Suitable for suspension cultures or monolayer human leukemic cells
- Manufactured with different formulations supplemented with Glutamine, Penicillin and Streptomycin Cell Culture Testedwith Phenol Red, HEPES, L-GlnSterile
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