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Item #:
90793928
10X PBS-T (Phosphate Buffered Saline with Tween-20), pH 7.4 - Sterile, 0.22um Filtered - 500 mL
83% of respondents would recommend this to a friend
Item #:
90793928
GMD 3529
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What Stands Out
Sterilization Assurance
This PBS-T solution is sterile and 0.22um filtered, ensuring the highest level of cleanliness for sensitive biological applications and research, reducing contamination risks effectively.
Convenient Volume
The 500 mL packaging provides ample volume for extensive laboratory experiments, minimizing downtime by reducing the frequency of repurchasing and replenishing essential reagents.
Optimal pH Balance
Formulated at pH 7.4, this PBS-T maintains physiological conditions ideal for biological assays, enhancing reliability and reproducibility in experiments that require precise buffer conditions.
Product Details
| Container Type | Bottle |
| Item Form | Liquid |
| Volume | 500 ml |
| Brand Name | UFC Bio |
| Manufacturer | UFC Bio |
| UPC | 791689523538 |
| Included Components | Sterile 10X PBS-T Buffer |
| Part Number | PBST-10X |
| Brand | UFC Bio |
| Item Volume | 500 ml |
| Package Information | Bottle |
| Item Weight | 1.1 lbs (500 grams) |
Who Should Buy?
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Laboratories
Ideal for use in immunological assays and washing steps due to its sterile and filtered properties.
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Cell Culture
Supports cell viability and provides a stable environment for various cell lines during in vitro experiments.
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Research Applications
Useful in various biochemical experiments, particularly in dilution and as a buffer for proteins and antibodies.
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Home Use
Not suitable for general household applications where laboratory-grade solutions are unnecessary and overly complex.
Product Description
10X PBS-T (Phosphate Buffered Saline with Tween-20), pH 7.4 - Sterile, 0.22um Filtered - 500 mL
Have any Query? Chat with us
Customer Questions & Answers
-
Question:
What is 10X PBS-T used for?
Answer: 10X PBS-T is primarily used in biological and biochemical laboratories as a buffer solution for various applications such as protein analyses, immunoassays, and cell culture. The presence of Tween-20 assists in minimizing non-specific binding in assays, enhancing the accuracy of results. Common scenarios include use in ELISA, Western Blotting, and sample dilutions. This versatility makes it an essential reagent in laboratories focused on research and diagnostics. -
Question:
How do I prepare 1X PBS-T from 10X PBS-T?
Answer: To prepare 1X PBS-T from 10X PBS-T, mix one part of 10X PBS-T with nine parts of sterile distilled water. This dilution results in a solution with a pH of 7.4, suitable for most laboratory applications. For instance, if you need 100 mL of 1X PBS-T, combine 10 mL of the 10X stock with 90 mL of distilled water. Proper preparation ensures the effectiveness of your experiments, enhancing the reliability of your data. -
Question:
Is 10X PBS-T sterile and filtered?
Answer: Yes, this 10X PBS-T solution is sterile and has been filtered through a 0.22 μm filter. This means that it is free from any microbial contamination, making it suitable for sensitive applications such as cell culture and other laboratory procedures where sterility is crucial. Using a sterile buffer helps prevent unwanted interactions in your experiments, maintaining the integrity of your samples. -
Question:
What are the storage requirements for 10X PBS-T?
Answer: 10X PBS-T should be stored at room temperature, away from direct sunlight and extreme temperatures. Once opened, it’s a good practice to minimize exposure to contaminants by using aseptic techniques. For long-term use, refrigeration is recommended. Proper storage prolongs the shelf-life and ensures that the quality of the solution remains intact, allowing for consistent results in your experiments. -
Question:
What makes Tween-20 an important component of PBS-T?
Answer: Tween-20 acts as a surfactant in PBS-T, which helps reduce non-specific binding when conducting assays. By minimizing background noise in immunoassays (like ELISA and Western Blotting), it enhances the specificity and sensitivity of detection methods. For instance, it can significantly improve signal clarity when staining proteins or antibodies. This is crucial in obtaining reliable data from experimental results. -
Question:
Can 10X PBS-T be used in cell culture applications?
Answer: Absolutely! 10X PBS-T is often utilized in various cell culture procedures, particularly for washing cells, diluting agents, and preparing solutions for assays. Its sterile nature is particularly beneficial when working with cultures sensitive to contamination. Researchers frequently use PBS-T as a base for making other media or reagents involved in experiments that require precise physiological conditions for optimal cell growth. -
Question:
Are there any known limitations for using 10X PBS-T?
Answer: While 10X PBS-T is very versatile, it may not be suitable for all types of reactions or experiments, particularly those requiring very low detergent concentrations. Additionally, certain sensitive proteins can be denatured by Tween-20. Therefore, it’s essential to evaluate your specific assay requirements. Researchers should consult their protocols or conduct preliminary tests to ensure compatibility with their specific applications. -
Question:
Is 10X PBS-T suitable for use in diagnostic testing?
Answer: Yes, 10X PBS-T is commonly used in clinical diagnostics due to its functionality as a buffer in assays like ELISA and Western Blotting. Its sterile and filtered nature makes it safe for these critical applications, preserving sample integrity. Healthcare laboratories utilize PBS-T for various tests to detect or quantify proteins and antibodies, making it invaluable in the diagnostic field. -
Question:
What is the pH level of 10X PBS-T, and why is it important?
Answer: The pH level of 10X PBS-T is 7.4, which is close to physiological pH. This is significant because maintaining physiological pH conditions is crucial for many biological experiments, especially when working with living cells and proteins. A consistent pH level ensures enzyme functionality and protein stability, which translates to more reproducible and reliable experimental outcomes. -
Question:
Where can I buy 10X PBS-T in The Gambia?
Answer: You can purchase 10X PBS-T (Phosphate Buffered Saline with Tween-20) from Ubuy. They offer a variety of laboratory supplies, and you can conveniently check their website for availability and additional details on this specific product. Ubuy provides a user-friendly shopping experience, making it easy to find what you need for your laboratory needs.
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